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Pepsinogen A Antibody (762015)

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Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Pepsinogen A was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) at 15 µg/mL overnight at 4 °C. Before incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
762015
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price
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Pepsinogen A Antibody (762015) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A4/PGA4
Ile16-Ala388
Accession # P00790
Specificity
Detects human Pepsinogen A4/PGA4 in direct ELISAs. Human PGA3 and PGA5 reactivity has not been demonstrated but is likely due to their high homology with human PGA4. In direct ELISAs, no cross-reactivity with recombinant human (rh) Pepsinogen C, rhCathepsin D, or rhCathepsin E is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PGA4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Pepsinogen A Antibody (762015)

  • EC 3.4.23
  • EC 3.4.23.1
  • FLJ58952
  • FLJ77962
  • pepsin A
  • pepsinogen 4, group I (pepsinogen A)
  • Pepsinogen A
  • pepsinogen A4
  • PGA3
  • PGA5

Background

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA3, PGA4, and PGA5 are human Pepsinogen A isozymogens that differ in sequence by 2‑4 amino acids (aa). Human pepsinogen A isozymogens share approximately 56% amino acid sequence identity with mouse and rat pepsinogen A isozymogens. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PGA4
Uniprot