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Pea3 Antibody

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Immunocytochemistry/ Immunofluorescence: Pea3 Antibody [NBP3-35390] - Immunofluorescence analysis of U-2 OS cells using Pea3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat ...read more
Western Blot: Pea3 Antibody [NBP3-35390] - Western blot analysis of various lysates using Pea3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry: Pea3 Antibody [NBP3-35390] - Immunohistochemistry analysis of paraffin-embedded Mouse skin using Pea3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with ...read more
Immunohistochemistry: Pea3 Antibody [NBP3-35390] - Immunohistochemistry analysis of paraffin-embedded Human skin using Pea3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with ...read more
Western Blot: Pea3 Antibody [NBP3-35390] - Western blot analysis of various lysates using Pea3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 ...read more
Immunohistochemistry: Pea3 Antibody [NBP3-35390] - Immunohistochemistry analysis of paraffin-embedded Rat skin using Pea3 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Pea3 Antibody Summary

Immunogen
Recombinant Protein corresponding to a sequence within amino acids 1-284 of human Pea3 (NP_001977.1).

Sequence:
KNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCEPEALFSLAFPDNQRPALKAEFDRPVSEEDTVPLSHLDESPAYLPELAGPAQPFGPKGGYSY
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ETV4
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.05% Proclin 300
Purity
Affinity purified

Alternate Names for Pea3 Antibody

  • Adenovirus E1A enhancer-binding protein
  • E1A-FE1A enhancer binding protein
  • E1AFETS translocation variant 4
  • ets variant 4
  • ets variant gene 4 (E1A enhancer binding protein, E1AF)
  • ets variant gene 4 (E1A enhancer-binding protein, E1AF)
  • EWS protein/E1A enhancer binding protein chimera
  • PEA3
  • PEAS3
  • Polyomavirus enhancer activator 3 homolog
  • polyomavirus enhancer activator-3
  • Protein PEA3

Background

Pea3, also known as ETS translocation variant 4, is an approximately 54 kDa, 484 amino acid protein, which is involved in transcriptional activation of adenovirus E1A gene, and is regulated by ERBB2, STK11, PLAG1, CTNNB1. Studies about the Pea3 protein are being done on diseases such as ovaria, pancreatic, gastric, breast, and prostate cancers. As well as peripheral primitive neuroectodermal tumors, carcinoma, adenoma, ewings sarcoma, squamous cell carcinoma, and oral cancers. The Pea3 protein has shown interaction with Taf7, EP300, SRF, HOXD4, STK11, and involvement in the ErbB2-ErbB3 Heterodimer pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ETV4