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Recombinant Human PDGF-AA Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Concentration
LYOPH

Order Details

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Recombinant Human PDGF-AA Protein Summary

Description
A disulfide-linked homodimeric protein containing two 126 amino acid polypeptide chains corresponding to PDGF-AA. However it migrates with an apparent molecular mass of 336 kDa in SDS-PAGE Source: E. coli

Amino Acid Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Details of Functionality
Biological activity measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for tis effect is 50-200 ng/mL in a fluorometric assay using a redox sensitive dye.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PDGFA
Purity
>95%, by SDS-PAGE
Endotoxin Note
Less than 0.1 EU/ug of PDGF-AA as determined by LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Briefly centrifuge vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to 0.1-1.0 mg/mL.

Alternate Names for Recombinant Human PDGF-AA Protein

  • PDGF1
  • PDGF-A
  • PDGFAA
  • PDGF-AA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PDGFA