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PDCD6 Antibody

Images

 
Western Blot: PDCD6 Antibody [NBP2-19741] - Sample (30 ug of whole cell lysate) A: HeLa nucleus 12% SDS PAGE gel, diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: PDCD6 Antibody [NBP2-19741] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PDCD6 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: PDCD6 Antibody [NBP2-19741] - Human colon carcinoma. PDCD6 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PDCD6 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PDCD6. The exact sequence is proprietary.
Localization
Nucleus membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Nucleus
Predicted Species
Mouse (99%), Rat (98%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDCD6
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (86%), Zebrafish (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for PDCD6 Antibody

  • ALG2
  • ALG-2
  • Apoptosis-linked gene 2 protein
  • apoptosis-linked gene-2
  • FLJ46208
  • MGC111017
  • MGC119050
  • MGC9123
  • PDCD6
  • PEF1B
  • Probable calcium-binding protein ALG-2
  • programmed cell death 6
  • programmed cell death protein 6

Background

This gene encodes a calcium-binding protein belonging to the penta-EF-hand protein family. Calcium binding is important for homodimerization and for conformational changes required for binding to other protein partners. This gene product participates in T cell receptor-, Fas-, and glucocorticoid-induced programmed cell death. In mice deficient for this gene product, however, apoptosis was not blocked suggesting this gene product is functionally redundant. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDCD6 Antibody (NBP2-19741) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDCD6
Uniprot