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Pax7 Antibody (SPM613) - Azide and BSA Free

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Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - Azide and BSA Free [NBP2-47923] - Human Rhabdomyosarcoma stained with PAX7 Monoclonal Antibody (SPM613).
Immunohistochemistry-Paraffin: Pax7 Antibody (SPM613) - Azide and BSA Free [NBP2-47923] - Human Rhabdomyosarcoma stained with PAX7 Monoclonal Antibody (SPM613).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow, IHC
Clone
SPM613
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47923AF350
Alexa Fluor 405
NBP2-47923AF405
Alexa Fluor 488
NBP2-47923AF488
Alexa Fluor 532
NBP2-47923AF532
Alexa Fluor 594
NBP2-47923AF594
Alexa Fluor 647
NBP2-47923AF647
Alexa Fluor 700
NBP2-47923AF700
Alexa Fluor 750
NBP2-47923AF750
Allophycocyanin
NBP2-47923APC
Biotin
NBP2-47923B
CoraFluor 1
NBP2-47923CL1
DyLight 350
NBP2-47923UV
DyLight 405
NBP2-47923V
DyLight 488
NBP2-47923G
DyLight 550
NBP2-47923R
DyLight 594
NBP2-47923DL594
DyLight 650
NBP2-47923C
DyLight 680
NBP2-47923FR
DyLight 755
NBP2-47923IR
FITC
NBP2-47923F
HRP
NBP2-47923H
Janelia Fluor 525
NBP2-47923JF525
Janelia Fluor 549
NBP2-47923JF549
Janelia Fluor 646
NBP2-47923JF646
Janelia Fluor 669
NBP2-47923JF669
PE
NBP2-47923PE
PerCP
NBP2-47923PCP
Unconjugated
NBP2-44589-0.1mg
mFluor Violet 450 SE
NBP2-47923MFV450
mFluor Violet 500 SE
NBP2-47923MFV500
mFluor Violet 610 SE
NBP2-47923MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44589-0.1mg
IHC-Prediluted
NBP2-48249
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Pax7 Antibody (SPM613) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44589).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant fragment (aa301-505) of human Pax7 protein (exact sequence is proprietary) (Uniprot: P23759)
Localization
Nuclear
Marker
Rhabdomyosarcoma Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PAX7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID: 32019766).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32019766).

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for Pax7 Antibody (SPM613) - Azide and BSA Free

  • FLJ37460
  • HUP1
  • paired box 7
  • paired box gene 7
  • paired box homeotic gene 7
  • paired box protein Pax-7
  • paired domain gene 7
  • PAX7 transcriptional factor
  • Pax7
  • PAX7B
  • RMS2

Background

PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Pax7 Antibody
(SPM613) - Azide and ...
NBP2-47923
Pax7 Antibody (SPM613) - Azide and BSA Free
Species: Hu, Mu
Applications: Flow, IHC

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Bioinformatics

Gene Symbol PAX7