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PARP4 Antibody

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Independent Antibodies: Western Blot: PARP4 Antibody [NBP1-06563] - Whole cell lysate from HeLa cells. vPARP was also immunoprecipitated by rabbit anti-vPARP antibody NBP1-06562, which recognizes an upstream ...read more
Immunohistochemistry-Paraffin: PARP4 Antibody [NBP1-06563] - Human colon carcinoma. Antibody: Affinity purified rabbit anti- vPARP used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

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PARP4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1674 to 1724 of human vault poly(ADP-ribose) polymerase using the numbering given in entry NP_006428.2 (GeneID 143).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARP4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PARP4 Antibody

  • 193 kDa vault protein
  • ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 1
  • ADPRTL1
  • ADPRTL1I-alpha-I-related
  • ARTD4
  • EC 2.4.2.30
  • H5 proline-rich
  • KIAA0177
  • p193
  • PARP4
  • PARP-4
  • PARPL
  • PARP-related
  • PARP-related/IalphaI-related H5/proline-rich
  • PH5P
  • poly (ADP-ribose) polymerase family, member 4
  • poly [ADP-ribose] polymerase 4
  • poly(ADP-ribose) synthetase
  • poly(ADP-ribosyl)transferase-like 1
  • Vault poly(ADP-ribose) polymerase
  • vault protein, 193-kDa
  • VAULT3
  • von Willebrand factor A domain containing 5C
  • VPARP
  • VPARPKIAA0177
  • VWA5C

Background

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PARP4
Entrez
Uniprot