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Park7/DJ-1 Antibody [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Bv, SyHa, ZeSpecies Glossary
Applications WB, Simple Western, Flow, Func, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-270AF350
Alexa Fluor 405
NB300-270AF405
Alexa Fluor 488
NB300-270AF488
Alexa Fluor 532
NB300-270AF532
Alexa Fluor 594
NB300-270AF594
Alexa Fluor 647
NB300-270AF647
Alexa Fluor 700
NB300-270AF700
Alexa Fluor 750
NB300-270AF750
Allophycocyanin
NB300-270APC
Biotin
NB300-270B
DyLight 350
NB300-270UV
DyLight 405
NB300-270V
DyLight 488
NB300-270G
DyLight 550
NB300-270R
DyLight 594
NB300-270DL594
DyLight 650
NB300-270C
DyLight 680
NB300-270FR
DyLight 755
NB300-270IR
FITC
NB300-270F
HRP
NB300-270H
Janelia Fluor 525
NB300-270JF525
Janelia Fluor 549
NB300-270JF549
Janelia Fluor 585
NB300-270JF585
Janelia Fluor 635
NB300-270JF635
Janelia Fluor 646
NB300-270JF646
Janelia Fluor 669
NB300-270JF669
PE
NB300-270PE
PerCP
NB300-270PCP
Unconjugated
NB300-270
mFluor Violet 450 SE
NB300-270MFV450
mFluor Violet 500 SE
NB300-270MFV500
mFluor Violet 610 SE
NB300-270MFV610
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Park7/DJ-1 Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A synthetic peptide corresponding to the C-terminus (within residues 150-189) of human Park7(DJ-1). [UniProt# Q99497]
Localization
Nucleus. Cytoplasm. Associated with mitochondria in some cells, particularly after oxidative stress. Detected in tau inclusions in brains from neurodegenerative disease patients.
Predicted Species
Bovine (100%), Zebrafish (100%), Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PARK7
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • In vitro assay
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to react with bovine, Zebra fish, and Golden Syrian hamster based on 100% sequence homology. Chicken reactivity reported in scientific literature (PMID: 24064392). Rat reactivity reported in scientific literature (PMID: 26419955)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Park7/DJ-1 Antibody [CoraFluor™ 1]

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

PARK7, also known as DJ-1, is a member of the peptidase C56 protein family. The PARK-7 protein is a positive regulator of androgen receptor-dependent transcription. It has also been reported to be a potential sensor for oxidative stress, a redox-sensitive chaperone, and a key protein for preventing neuronal oxidative stress and cell death.

PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.
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Bioinformatics

Gene Symbol PARK7