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Park7/DJ-1 Antibody (925805R) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MEF mouse embryonic feeder cells, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MEF mouse embryonic feeder cells, and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Park7/DJ-1 Monoclonal Antibody (Catalog # MAB39951) at 10 µg/mL for 3 hours at room ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ...read more
Recombinant Human Park7/DJ‑1 protein was serially diluted 2-fold and captured by Mouse Anti-Human Park7/DJ‑1 Monoclonal Antibody (Catalog # MAB39952) coated on a Clear Polystyrene Microplate (Catalog # ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, KO
Clone
925805R
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB39951-SP
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Park7/DJ-1 Antibody (925805R) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
E. coli-derived recombinant human PARK-7
Ala2-Asp189
Accession # Q99497
Specificity
Detects human, mouse, and rat PARK-7 in Western blots. In sandwich immunoassays, it is specific for human PARK-7.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PARK7
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Park7/DJ-1 Antibody (925805R) [Unconjugated]

  • DJ1
  • DJ-1
  • DJ1FLJ34360
  • EC 3.4
  • FLJ27376
  • FLJ92274
  • Oncogene DJ1
  • Park7
  • Parkinson disease (autosomal recessive, early onset) 7
  • Parkinson disease protein 7
  • protein DJ-1

Background

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease. Human and mouse Park7 share 92% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Park7/DJ-1
Antibody (925805R) [U ...
MAB39951
Park7/DJ-1 Antibody (925805R) [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, KO

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Blogs on Park7/DJ-1.

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Park7/DJ-1: A Reliable Biomarker for Parkinson's Disease?
The product of the Parkinson's disease 7 (Park7/DJ-1) gene belongs to the peptidase C56 family of proteins and appears to have two transcriptional variants. It is a positive regulator of androgen receptor-dependent transcription, and some evidence sug...  Read full blog post.
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Bioinformatics

Gene Symbol PARK7
Uniprot