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PAC1R Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining in human cerebral cortex and pancreas tissues using anti-ADCYAP1R1 antibody. Corresponding ADCYAP1R1 RNA-seq data are ...read more
Western Blot: PAC1R Antibody [NBP2-47340] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP2-47340] - Staining of human cell line SH-SY5Y shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining of human cerebral cortex shows high expression.
Immunohistochemistry-Paraffin: PAC1R Antibody [NBP2-47340] - Staining of human pancreas shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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PAC1R Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SDCIFKKEQAMCLEKIQRANELMGFNDSSPGCPGMWD
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADCYAP1R1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
PAC1R Protein (NBP2-47340PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PAC1R Antibody

  • ADCYAP1R1
  • adenylate cyclase activating polypeptide 1 (pituitary) receptor type 1
  • adenylate cyclase activating polypeptide 1 (pituitary) receptor type I
  • PAC1R
  • PACAP type I receptor
  • PACAPR
  • PACAP-R1
  • PACAP-R-1
  • PACAPRI
  • PACAPRPAC1
  • pituitary adenylate cyclase activating polypeptide 1 receptor type I Hiphop
  • pituitary adenylate cyclase-activating polypeptide type I receptor

Background

The pituitary adenylate cyclase-activating peptide receptor (PAC1) is a member of a family of three G-protein coupled receptors which mediate VIP (vasoactive intestinal peptide) and PACAP (pituitary adenlyate cyclase activating peptide) function in a variety of tissues. PAC1 demonstrates a higher affinity for PACAP than VIP. Expression of the VIP and VACAP receptors has been linked to the presence of human tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional PAC1R Products

Array NBP2-47340

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Bioinformatics

Gene Symbol ADCYAP1R1