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p70 S6 Kinase/S6K Antibody (OTI1G4) [PE/Cy5.5]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Cy5.5

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73209AF350
Alexa Fluor 405
NBP2-73209AF405
Alexa Fluor 488
NBP2-73209AF488
Alexa Fluor 532
NBP2-73209AF532
Alexa Fluor 594
NBP2-73209AF594
Alexa Fluor 647
NBP2-73209AF647
Alexa Fluor 700
NBP2-73209AF700
Alexa Fluor 750
NBP2-73209AF750
Allophycocyanin
NBP2-73209APC
Allophycocyanin/Cy7
NBP2-73209APCCY7
Biotin
NBP2-73209B
CoraFluor 1
NBP2-73209CL1
DyLight 350
NBP2-73209UV
DyLight 405
NBP2-73209V
DyLight 488
NBP2-73209G
DyLight 550
NBP2-73209R
DyLight 594
NBP2-73209DL594
DyLight 650
NBP2-73209C
DyLight 680
NBP2-73209FR
DyLight 755
NBP2-73209IR
FITC
NBP2-73209F
HRP
NBP2-73209H
Janelia Fluor 525
NBP2-73209JF525
Janelia Fluor 549
NBP2-73209JF549
Janelia Fluor 646
NBP2-73209JF646
Janelia Fluor 669
NBP2-73209JF669
PE
NBP2-73209PE
PE/Atto594
NBP2-73209PEATT594
PE/Cy7
NBP2-73209PECY7
PerCP
NBP2-73209PCP
Unconjugated
NBP2-00754
mFluor Violet 450 SE
NBP2-73209MFV450
mFluor Violet 500 SE
NBP2-73209MFV500
mFluor Violet 610 SE
NBP2-73209MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00754
Azide and BSA Free
NBP2-73209
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p70 S6 Kinase/S6K Antibody (OTI1G4) [PE/Cy5.5] Summary

Immunogen
Full length recombinant protein of human p70 S6 Kinase/S6K (NP_003152) produced in HEK293T cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RPS6KB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for p70 S6 Kinase/S6K Antibody (OTI1G4) [PE/Cy5.5]

  • EC 2.7.11
  • EC 2.7.11.1
  • p70 ribosomal S6 kinase alpha
  • p70 S6 kinase alpha
  • p70 S6 Kinase
  • p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1
  • p70 S6 kinase, alpha 2
  • p70 S6KA
  • p70 S6K-alpha
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • P70S6K1
  • PS6K
  • ribosomal protein S6 kinase beta-1
  • Ribosomal protein S6 kinase I
  • ribosomal protein S6 kinase, 70kD, polypeptide 1
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • RPS6KB1
  • S6K
  • S6K1
  • S6K1p70-S6K 1
  • S6K-beta-1
  • serine/threonine kinase 14 alpha
  • Serine/threonine-protein kinase 14A
  • STK14A

Background

S6K encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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S6K - a serine/threonine kinase with diverse roles in cell survival and cell cycle progression
S6K is a serine/threonine kinase that is a member of the ribosomal S6 kinase (RSK) family. S6K exists in two main isoforms, S6K1 and S6K2, which can also be alternatively spliced to produce different splice forms. S6K1 has two major splicing produ...  Read full blog post.
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Bioinformatics

Gene Symbol RPS6KB1