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P4HA1 Antibody (4F1)

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Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] - Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L)
Immunocytochemistry/ Immunofluorescence: P4HA1 Antibody (4F1) [NBP3-43012] - Immunofluorescent analysis of (-20℃ Ethanol ) fixed PC-3 cells using (P4HA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated ...read more
Western Blot: P4HA1 Antibody (4F1) [NBP3-43012] - PC-3 cells were subjected to SDS PAGE followed by western blot with (P4HA1 antibody) at dilution of 1:1000
Immunohistochemistry: P4HA1 Antibody (4F1) [NBP3-43012] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (P4HA1 Antibody) at dilution of 1:500 heat mediated antigen retrieved with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
4F1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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P4HA1 Antibody (4F1) Summary

Immunogen
prolyl 4-hydroxylase, alpha polypeptide I - (Uniprot# P13674)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
P4HA1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for P4HA1 Antibody (4F1)

  • 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase)
  • 2-oxoglutarate-4-dioxygenase subunit alpha-1
  • 4-PH Alpha-1
  • alpha polypeptide I
  • EC 1.14.11.2
  • P4HA
  • P4HA1
  • Procollagen-proline
  • prolyl 4-hydroxylase, alpha polypeptide I

Background

P4HA1 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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P4HA1 Antibody (4F1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol P4HA1