![Immunoprecipitation: P2Y2 Antibody [NBP2-15060] - Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 ug of P2Y2 antibody [N3C3]. Western blot analysis was performed using P2Y2 antibody [N3C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/P2Y2-Antibody-Immunoprecipitation-NBP2-15060-img0004.jpg "Immunoprecipitation: P2Y2 Antibody [NBP2-15060] - Immunoprecipitation of P2Y2 protein from 293T whole cell extracts using 5 ug of P2Y2 antibody [N3C3]. Western blot analysis was performed using P2Y2 antibody [N3C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human P2Y2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | P2RY2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for P2Y2 Antibody (NBP2-15060)Find related products by research area.
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P2Y2 (P2Y purinoceptor 2, ATP receptor) The protein P2Y2 is a G-protein coupled metabotropic receptor that belongs to a larger family consisting of several receptor subtypes that each has a different pharmacological selectivity for various adenosine and uridine nucleotides. (This selecti... Read full blog post. |
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![Western Blot: P2Y2 Antibody [NBP2-15060] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-910202420122044.jpg "Western Blot: P2Y2 Antibody [NBP2-15060] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242011451.jpg "Immunocytochemistry/ Immunofluorescence: P2Y2 Antibody [NBP2-15060] - P2Y2 antibody [N3C3] detects P2Y2 protein at cell membrane by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: P2Y2 stained by P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:500.Blue: Hoechst 33342 staining.")
![Western Blot: P2Y2 Antibody [NBP2-15060] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15060_rabbit-polyclonal-p2y2-antibody-91020242001015.jpg "Western Blot: P2Y2 Antibody [NBP2-15060] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y2 antibody [N3C3] (NBP2-15060) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")