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Mouse p27/Kip1 ELISA Kit (Colorimetric)

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ELISA: Mouse p27/Kip1 ELISA Kit (Colorimetric) [NBP2-75208] - Samples were spiked with high concentrations of Mouse p27/Kip1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Mouse p27/Kip1 ELISA Kit (Colorimetric) [NBP2-75208] - Samples were spiked with high concentrations of Mouse p27/Kip1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Mouse p27/Kip1 ELISA Kit (Colorimetric) [NBP2-75208] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL

Order Details

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Mouse p27/Kip1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse CDKN1B in samples. No significant cross-reactivity or interference between Mouse CDKN1B and analogues was observed.
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.43%
Intra-Assay
CV% < 5.24%
Spike Recovery
87-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CDKN1B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse p27/Kip1 ELISA Kit (Colorimetric)

  • CDKN1B
  • CDKN4
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • cyclin-dependent kinase inhibitor 1B
  • Cyclin-dependent kinase inhibitor p27
  • Kip1
  • KIP1P27KIP1
  • MEN1B
  • MEN4
  • p27
  • p27Kip1

Background

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Mouse p27/Kip1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CDKN1B