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p21/CIP1/CDKN1A Antibody (WA-1) [Alexa Fluor® 700]

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Product Details

Summary
Reactivity Hu, Mu, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
WA-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 700

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-33084AF350
Alexa Fluor 405
NBP2-33084AF405
Alexa Fluor 488
NBP2-33084AF488
Alexa Fluor 532
NBP2-33084AF532
Alexa Fluor 594
NBP2-33084AF594
Alexa Fluor 647
NBP2-33084AF647
Alexa Fluor 750
NBP2-33084AF750
Allophycocyanin
NBP2-33084APC
Biotin
NBP2-33084B
DyLight 350
NBP2-33084UV
DyLight 405
NBP2-33084V
DyLight 488
NBP2-33084G
DyLight 550
NBP2-33084R
DyLight 594
NBP2-33084DL594
DyLight 650
NBP2-33084C
DyLight 680
NBP2-33084FR
DyLight 755
NBP2-33084IR
FITC
NBP2-33084F
HRP
NBP2-33084H
Janelia Fluor 549
NBP2-33084JF549
Janelia Fluor 646
NBP2-33084JF646
PE
NBP2-33084PE
PerCP
NBP2-33084PCP
Unconjugated
NBP2-29463
mFluor Violet 450 SE
NBP2-33084MFV450
mFluor Violet 500 SE
NBP2-33084MFV500
mFluor Violet 610 SE
NBP2-33084MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-29463
Azide and BSA Free
NBP2-33084
IHC-Prediluted
NBP2-44524
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p21/CIP1/CDKN1A Antibody (WA-1) [Alexa Fluor® 700] Summary

Immunogen
Human recombinant p21/CIP1/CDKN1A protein (Uniprot: P38936 )
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, monoclonal antibody WA-1 is useful either as a solid phase or for detection of p21 protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDKN1A
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p21/CIP1/CDKN1A Antibody (WA-1) [Alexa Fluor® 700]

  • CAP20cyclin-dependent kinase inhibitor 1
  • CDK-interacting protein 1
  • CDKN1A
  • CDKN1melanoma differentiation associated protein 6
  • CIP1
  • CIP1WAF1CDK-interaction protein 1
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • MDA6
  • MDA-6
  • Melanoma differentiation-associated protein 6
  • p21
  • p21CIP1
  • p21Cip1/Waf1
  • PIC1
  • SDI1DNA synthesis inhibitor
  • wild-type p53-activated fragment 1

Background

Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and over expression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDKN1A