![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.](http://images.novusbio.com/fullsize/P-Selectin-CD62P-Antibody-Psel-KO-2-7-DyLight-488-Flow-Cytometry-NB100-65392G-img0004.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.")
| Reactivity | HuSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP |
| Clone | Psel.KO.2.7 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 488 |
| Description | This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet. |
| Immunogen | P-selectin transfected 300.19 cells |
| Localization | Membrane; single pass type I membrane protein. |
| Specificity | NB100-65392 recognizes the P-Selectin cell surface antigen, a 140kD glycoprotein. P-Selectin is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SELP |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
![Immunohistochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](https://images.novusbio.com/images2/E-Selectin_BBA16_Immunohistochemistry_6938.jpg)
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![Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]](https://images.novusbio.com/images/antibody/bba24_human-l-selectin-cd62l-mab-cl-4g8-flow-cytometry-822024174319..jpg)
![Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_10287.jpg)
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![Immunohistochemistry CD31/PECAM-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/CD31_AF3628_Immunohistochemistry_21486.jpg)
![P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488]")
| Images | Ratings | Applications | Species | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
Flow | Other | 11/01/2013 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SELP |
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).](http://images.novusbio.com/fullsize/P-Selectin-CD62P-Antibody-Psel-KO-2-7-DyLight-488-Flow-Cytometry-NB100-65392G-img0003.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Thrombin stimulated equine platelets (solid line) and unstimulated platelets (dashed line) labeled with anti-CD62P (Psel.KO.2.7) compared to equine platelets labeled with murine IgG-DyLight 488 isotype control (gray fill).")
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.](http://images.novusbio.com/fullsize/P-Selectin-CD62P-Antibody-Psel-KO-2-7-DyLight-488-Flow-Cytometry-NB100-65392G-img0005.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Differential CD62P (P-selectin) expression of equine platelets in response to varying agonist treatments (Control (1), Thrombin (2), Convulxin (3), Ionophore A23187 (4)). All samples were first gated on CD61 positive events.")
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65392g_mouse-monoclonal-p-selectin-cd62p-antibody-psel-ko-2-7-dylight-488-31020241682190.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65392g_mouse-monoclonal-p-selectin-cd62p-antibody-psel-ko-2-7-dylight-488-31020241682170.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-65392g_mouse-monoclonal-p-selectin-cd62p-antibody-psel-ko-2-7-dylight-488-310202415535190.jpg "Flow Cytometry: P-Selectin/CD62P Antibody (Psel.KO.2.7) [DyLight 488] [NB100-65392G] - Flow cytometry gating strategy for quantification of platelet-derived microparticles in equine platelet samples.A: Platelet events in citrate-anticoagulated platelet-rich plasma were identified & gated as CD41-positive cells (R1 region) in a CD41 fluorescence versus forward scatter (FSC) dotplot. The R1 region or gate was established on an isotype control for the CD41 antibody. Representative image from platelets exposed to the RacL11 strain of EHV-1 at 1 plaque forming unit (PFU)/cell. B: Platelet-derived microparticles (PDMPs) were defined as small events (<101 log FSC units) positive for Annexin V & CD41. The PDMP percentage was obtained from the lower right quadrant of an Annexin V fluorescence versus FSC dotplot of the R1 gate (CD41-positive events), with the quadrants being defined on a negative sample in which 1 mM EDTA was added to the buffer with Annexin V. The PDMP percentage was 0.1% in this representative image of platelets exposed to rabbit kidney 13 (RK) cell lysate at an equivalent volume to 1 PFU/cell (mock-infected negative control). The events in the upper left & right quadrants are platelets that are negative (94.0%) & positive for Annexin V (0.9%), respectively. C: Representative image of PDMP quantification in platelets exposed to RacL11 at 1 PFU/cell. In this sample, there are 12.1% PDMP (lower right quadrant) & 22.1% of platelets are weakly positive for Annexin V (upper right quadrant). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25905776), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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