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OSTM1 Antibody

Images

 
Western Blot: OSTM1 Antibody [NBP1-81829] - Analysis in control (vector only transfected HEK293T lysate) and OSTM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: OSTM1 Antibody [NBP1-81829] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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OSTM1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: REAYKTLSSLYSEMQKMNELENKAEPGTHLCIDVEDAMNITRKLWSRTFNCSVP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OSTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
OSTM1 Protein (NBP1-81829PEP)
Publications
Read Publications using NBP1-81829.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (89%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for OSTM1 Antibody

  • GIPN
  • GLGAIP-interacting protein N terminus
  • grey-lethal osteopetrosis
  • HSPC019
  • OPTB5
  • osteopetrosis associated transmembrane protein 1
  • osteopetrosis-associated transmembrane protein 1

Background

OSTM1 encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OSTM1 Antibody (NBP1-81829)(2)

Reviews for OSTM1 Antibody (NBP1-81829) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OSTM1 Antibody (NBP1-81829). (Showing 1 - 1 of 1 FAQs).

  1. Does the OSTM1 antibody (NBP1-81829) work on murine tissues or proteins in western blot and immunohistochemistry (frozen sections)?
    • Our OSTM1 antibody (NBP1-81829) has been validated in Human samples for WB/IHC-P applications only. There is a considerable similarity in the immunogen sequence used for this product when compared the same to the OSTM1 sequence of mouse protein, so that there is a pretty good chance of cross-reactivity. Therefore, you may optimize the best suitable conditions for the assay from your testing, and we would be more than happy to provide you our troubleshooting/technical feedback if you come up with any problems in generating your desired outcome. If you decide to test OSTM1 (NBP1-81829) in Mouse/IHC-Fr, and share your results/review feedback with us, please consider our Innovator's Reward program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol OSTM1