Osteopontin/OPN Antibody (1B20) - Azide and BSA Free Summary
Immunogen |
Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451]. |
Localization |
Secreted. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
SPP1 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:50
- Immunohistochemistry 1:100
- Immunohistochemistry-Frozen reported in scientific literature (PMID 24269728)
- Immunohistochemistry-Paraffin 1:100
- Immunoprecipitation 1:10-1:500
- Simple Western 1:20
- Western Blot 1:1000
|
Application Notes |
In Western blot multiple bands can be seen due to glycosylation and phosphorylation of the protein.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.4) |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for Osteopontin/OPN Antibody (1B20) - Azide and BSA Free
Background
The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood, and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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