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Osteopontin/OPN Antibody (1B20) [DyLight 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP
Clone
1B20
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

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Osteopontin/OPN Antibody (1B20) [DyLight 405] Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human Osteopontin [Swiss-Prot# P10451].
Localization
Secreted.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, Rat and Rabbit. Rabbit reactivity reported in scientific literature (PMID:33001866). Use in Mouse reported in scientific literature (PMID:33580126).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Osteopontin/OPN Antibody (1B20) [DyLight 405]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

The matricellular protein osteopontin binds to cell surface receptors and is secreted into many body fluids including milk, blood and urine, depending on the organ of origin. This makes osteopontin an ideal candidate for being a biomarker as the secreted form is easily obtained in throwaway fluids, and mimics the cellular environment from which it is released. Osteopontin is important in immune responses and inflammation as well as bone generation and remodeling. In autistic children, serum levels of osteopontin are correlated to the severity of disease, probably due to a brain inflammation pattern in these children. In aortic valve sclerosis and stenosis, increased levels of secreted osteopontin are also noted. Osteopontin has also been suggested as a cancer biomarker, since it is associated with tumor formation, progression and metastasis. In bone and tooth formation osteopontin is known to be a negative regulator of parathyroid hormone-related protein receptor, which induces osteogenesis. Without appropriate levels of osteopontin, bone growth continues unregulated, and leads to specific bone cancers. In short, osteopontin is a strong marker for bone growth, inflammation and certain cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Osteopontin/OPN Antibody (NB110-89062V) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPP1