Novus Biologicals products are now on bio-techne.com

OGDH Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: OGDH Antibody [NBP2-19622] - OGDH antibody detects OGDH protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ...read more
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse heart. OGDH stained by OGDH antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: OGDH Antibody [NBP2-19622] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:5000.
Western Blot: OGDH Antibody [NBP2-19622] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and blotted with OGDH antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) ...read more
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Cal27 xenograft, using OGDH antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse muscle. OGDH antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: OGDH Antibody [NBP2-19622] - Mouse muscle. OGDH stained by OGDH antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: OGDH Antibody [NBP2-19622] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [C2C3], C-term (NBP2-19622) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

OGDH Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Rat (93%), Bovine (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OGDH
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
Theoretical MW
116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (87%), Xenopus laevis (84%),

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for OGDH Antibody

  • 2-oxoglutarate dehydrogenase complex component E1
  • 2-oxoglutarate dehydrogenase, mitochondrial
  • AKGDH
  • alpha KGD
  • alpha-KGD
  • E1k
  • KGD1
  • OGDC
  • OGDC-E1
  • OGDH
  • OGDH2
  • oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
  • oxoglutarate decarboxylase
  • oxoglutarate dehydrogenase (succinyl-transferring)

Background

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
NBP2-94660
Species: Hu, Mu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-88347
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-34065
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
H00011026-M01
Species: Hu
Applications: ELISA, WB
NBP1-31715
Species: Hu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84079
Species: Hu
Applications: IHC, IHC-P, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP3-05078
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-32398
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
NBP1-89559
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87308
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
H00003033-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NBP1-85630
Species: Hu
Applications: IHC, IHC-P, WB
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for OGDH Antibody (NBP2-19622) (0)

There are no publications for OGDH Antibody (NBP2-19622).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OGDH Antibody (NBP2-19622) (0)

There are no reviews for OGDH Antibody (NBP2-19622). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OGDH Antibody (NBP2-19622) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional OGDH Products

Research Areas for OGDH Antibody (NBP2-19622)

Find related products by research area.

Blogs on OGDH

There are no specific blogs for OGDH, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our OGDH Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol OGDH
Uniprot