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Oct-4A Antibody (653108) [Allophycocyanin]

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BG01V human embryonic stem cells were stained with Mouse Anti-Human Oct-4A APC-conjugated Monoclonal Antibody (Catalog # IC6344A, filled histogram) or isotype control antibody (IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
BG01V human embryonic stem cells were stained with Mouse Anti-Human Oct-4A APC-conjugated Monoclonal Antibody (Catalog # IC6344A, filled histogram) or isotype control antibody (IC003A, open histogram). To facilitate ...read more
iPSCs were stained with Mouse Anti-Human Oct‑4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
653108
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC17591U-100UG
Alexa Fluor 405
IC17591V-100UG
Alexa Fluor 488
IC17591G-100UG
Alexa Fluor 594
IC17591T-100UG
Alexa Fluor 647
IC17591R-100UG
Alexa Fluor 700
IC17591N-100UG
Alexa Fluor 750
IC17591S-100UG
Unconjugated
MAB17591
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB17591
Carrier Free
MAB17591
Carrier Free
MAB17591-SP
Carrier Free
IC17591V-100UG
Carrier Free
IC17591U-100UG
Carrier Free
IC17591T-100UG
Carrier Free
IC17591S-100UG
Carrier Free
IC17591R-100UG
Carrier Free
IC17591N-100UG
Carrier Free
IC17591G-100UG
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Oct-4A Antibody (653108) [Allophycocyanin] Summary

Specificity
Detects human Oct-4A in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
POU5F1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Oct-4A Antibody (653108) [Allophycocyanin]

  • OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1
  • Oct4A
  • Oct-4A

Background

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POU5F1
Uniprot