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OAT Antibody

Images

 
Western Blot: OAT Antibody [NBP1-32390] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was ...read more
Immunocytochemistry/ Immunofluorescence: OAT Antibody [NBP1-32390] - OAT antibody detects OAT protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: OAT ...read more
Immunohistochemistry-Paraffin: OAT Antibody [NBP1-32390] - Human gastric cancer, using OAT antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: OAT Antibody [NBP1-32390] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: OAT Antibody [NBP1-32390] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OAT antibody [N1C3] (NBP1-32390) ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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OAT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human OAT. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Rat (93%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OAT
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (86%) and Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for OAT Antibody

  • GACR
  • HOGA
  • OAT ornithine aminotransferase
  • OATASE
  • OKT

Background

OAT, also known as mitochondrial enzyme ornithine aminotransferase, is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. ( L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid.) Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Recombinant OAT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol OAT