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Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]

 (19 Publications)
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Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2156) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal ...read more
Nurr1/NGFI‑B beta /NR4A2 was detected in immersion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Human/Mouse Nurr1/NGFI‑B beta /NR4A2 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF2156-SP
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Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse Nurr1/NGFI‑B beta /NR4A2
Val332-Lys558
Accession # Q06219
Specificity
Detects human and mouse Nurr1/NGFI‑B beta /NR4A2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NR4A2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2156 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]

  • human homolog of
  • NGFI-B beta
  • NOT
  • NR4A2
  • nuclear receptor subfamily 4, group A, member 2
  • Nurr1
  • orphan nuclear receptor NR4A2
  • Orphan nuclear receptor NURR1
  • T-cell nuclear receptor NOT
  • TINUR
  • transcriptionally inducible nuclear receptor related 1
  • Transcriptionally-inducible nuclear receptor

Background

Nurr1 belongs to the nerve growth factor-induced clone B subfamily of nuclear receptor superfamily and is designated NR4A2 in the Unified Nomenclature System for NRs. Nurr1 can function as a ligand-independent transcription factor. Nurr1 also heterodimerizes with retinoid X receptors (RXRs) to function as ligand-regulated transcription factor. Nurr1 is important for specification of dopaminergic neurons that degenerate in Parkinson’s disease. Human and mouse Nurr1 share 99% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nurr1/NGFI-B beta/NR4A2 Antibody (AF2156)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(1)
All Applications
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Human
(2)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF2156 Applications Species
Shaker, T;Dagpa, GJ;Cattaud, V;Marriott, BA;Sultan, M;Almokdad, M;Jackson, J; A simple and reliable method for claustrum localization across age in mice Molecular brain 2024-02-17 [PMID: 38368400]
Salamon I, Park Y, Mi�ki? T et al. Celf4 controls mRNA translation underlying synaptic development in the prenatal mammalian neocortex Nat Commun 2023-09-27 [PMID: 37758766]
Galazo, MJ;Sweetser, DA;Macklis, JD; Tle4 controls both developmental acquisition and early post-natal maturation of corticothalamic projection neuron identity Cell reports 2023-08-09 [PMID: 37561632] (IHC, Mouse) IHC Mouse
Martin Munz, Arjun Bharioke, Georg Kosche, Verónica Moreno-Juan, Alexandra Brignall, Tiago M. Rodrigues et al. Pyramidal neurons form active, transient, multilayered circuits perturbed by autism-associated mutations at the inception of neocortex Cell 2023-04-27 [PMID: 37071993]
Flavia Correa Turcato, Elliot Wegman, Tao Lu, Nathan Ferguson, Yu Luo Dopaminergic neurons are not a major Sonic hedgehog ligand source for striatal cholinergic or PV interneurons iScience 2022-11-18 [PMID: 36281454]
Anna Hoerder-Suabedissen, Gabriel Ocana-Santero, Thomas H Draper, Sophie A Scott, Jesse G Kimani, Andrew M Shelton et al. Temporal origin of mouse claustrum and development of its cortical projections Cerebral Cortex 2023-03-21 [PMID: 36104852]
F Luo, J Wang, Z Zhang, Z You, A Bedolla, F Okwubido-W, LF Huang, J Silver, Y Luo Inhibition of CSPG receptor PTPsigma promotes migration of newly born neuroblasts, axonal sprouting, and recovery from stroke Oncogene, 2022-07-26;40(4):111137. 2022-07-26 [PMID: 35905716] (IHC, Mouse) IHC Mouse
M Shibata, K Pattabiram, B Lorente-Ga, D Andrijevic, SK Kim, N Kaur, SK Muchnik, X Xing, G Santpere, AMM Sousa, N Sestan Regulation of prefrontal patterning and connectivity by retinoic acid Nature, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599305] (IHC, Human, Mouse) IHC Human, Mouse
Evangel Kummari, Shirley X. Guo-Ross, Heath S. Partington, Jennifer Makenzie Nutter, Jeffrey B. Eells Quantitative Immunohistochemistry to Measure Regional Expression of Nurr1 in the Brain and the Effect of the Nurr1 Heterozygous Genotype Frontiers in Neuroanatomy 2021-04-30 [PMID: 33994958]
Shin-Hwa Tsai, Chih-Yu Tsao, Li-Jen Lee Altered White Matter and Layer VIb Neurons in Heterozygous Disc1 Mutant, a Mouse Model of Schizophrenia Frontiers in Neuroanatomy 2020-12-15 [PMID: 33384588]
Show All 19 Publications.

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Bioinformatics

Gene Symbol NR4A2
Uniprot