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Nucleostemin Antibody

Images

 
Western Blot: Nucleostemin Antibody [NBP2-38325] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human skin.
Immunohistochemistry: Nucleostemin Antibody [NBP2-38325] - Staining of human skin shows strong nucleolar positivity in epidermal cells.
Independent Antibodies: Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney, liver, skin and testis using Anti-GNL3 antibody NBP2-38325 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human testis.
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human liver.
Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

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Nucleostemin Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: KQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNL3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Nucleostemin Recombinant Protein Antigen (NBP2-38325PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Nucleostemin Antibody

  • C77032
  • E2IG3NNP47
  • estradiol-induced nucleotide binding protein
  • GNL3
  • guanine nucleotide binding protein-like 3 (nucleolar)
  • guanine nucleotide-binding protein-like 3
  • MGC800
  • Novel nucleolar protein 47
  • NSE2-induced gene 3 protein
  • Nucleolar GTP-binding protein 3
  • Nucleostemin

Background

Nucleostemin is a protein found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GNL3
Uniprot