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![Western Blot: Nucleostemin Antibody [NBP2-38326] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-38326-img0003.jpg "Western Blot: Nucleostemin Antibody [NBP2-38326] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-38326] - Staining of human cell line U-2 OS shows localization to nucleoli & nuclear bodies.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38326-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human testis.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0008.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NBP2-38326] - Staining of human oral mucosa shows strong nuclear/ nucleolar positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NBP2-38326-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human kidney, liver, skin and testis using Anti-GNL3 antibody NBP2-38326 (A) shows similar protein distribution across tissues to independent antibody NBP2-38325 (B).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human skin.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human kidney.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0007.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38326] - Staining of human liver.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38326-img0009.jpg)
![Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NB100-1569-img0005.jpg "Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by western blot. Samples:Whole cell lysate (50 ug) from TMCK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0007.jpg)
![Western Blot: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot. Samples:Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GNL3 antibody NB100-1569 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NB100-1569-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NB100-1569] - Human skin carcinoma. Antibody used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NB100-1569-img0002.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0003.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NB100-1569] - Detection of mouse GNL3 by immunohistochemistry. Sample: FFPE section of mouse CT26 colon carcinoma. Antibody: Affinity purified rabbit anti-GNL3 (NB100-1569). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1569-img0008.jpg)
![Immunoprecipitation: Nucleostemin Antibody [NB100-1569] - Detection of human GNL3 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GNL3 antibody NB100-1569(lot NB100-1569-3) used for IP at 6 ug per reaction. GNL3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1569-2). For blotting immunoprecipitated GNL3, NB100-1569 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunoprecipitation-NB100-1569-img0006.jpg)
![Western Blot: Nucleostemin Antibody [NBP2-38325] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-38325-img0003.jpg "Western Blot: Nucleostemin Antibody [NBP2-38325] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human skin.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0007.jpg)
![Immunohistochemistry: Nucleostemin Antibody [NBP2-38325] - Staining of human skin shows strong nucleolar positivity in epidermal cells.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NBP2-38325-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney, liver, skin and testis using Anti-GNL3 antibody NBP2-38325 (A) shows similar protein distribution across tissues to independent antibody NBP2-38326 (B).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0004.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human testis.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0005.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human liver.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0006.jpg)
![Immunohistochemistry-Paraffin: Nucleostemin Antibody [NBP2-38325] - Staining of human kidney.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-Paraffin-NBP2-38325-img0008.jpg)
![Western Blot: Nucleostemin Antibody [NB100-1568] - Detection of Human and Mouse GNL3 on HeLa whole cell lysate using NB100-1568. GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies NB100-1569 and another GNL3 Ab.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NB100-1568-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Nucleostemin Antibody [NB100-1568] - Detection of Human and Mouse GNL3 on HeLa whole cell lysate using NB100-1568. GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies NB100-1569 and another GNL3 Ab.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: Nucleostemin Antibody [NB100-1568] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunohistochemistry-NB100-1568-img0003.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0004.jpg "Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mouse mAb against NIH3T3 (1) and PC-3 (2) cell lysate.")
![Flow Cytometry: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Flow cytometric analysis of Jurkat cells using GNL3 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Flow-Cytometry-NBP2-37410-img0005.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against human GNL3 recombinant protein. (Expected MW is 51.9 kDa)](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0002.jpg)
![Western Blot: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Western blot analysis using GNL3 mAb against HEK293 (1) and GNL3 (AA: 1-226)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-Western-Blot-NBP2-37410-img0003.jpg)
![ELISA: Nucleostemin Antibody (2C8D5) [NBP2-37410] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-2C8D5-ELISA-NBP2-37410-img0001.jpg)
![Western Blot: Nucleostemin Antibody [NBP2-98743] - Anti-Nucleostemin rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Western-Blot-NBP2-98743-img0002.jpg "Western Blot: Nucleostemin Antibody [NBP2-98743] - Anti-Nucleostemin rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 62 kDa (We are unsure as to the identity of these extra bands).")
![Immunocytochemistry/Immunofluorescence: Nucleostemin Antibody [NBP2-98743] - Immunofluorescence staining of Nucleostemin in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Nucleostemin polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green)Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize/Nucleostemin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98743-img0001.jpg)
![Western Blot: Nucleostemin Overexpression Lysate (Adult Normal) [NBL1-11183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleostemin.](http://images.novusbio.com/fullsize/Nucleostemin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11183-img0002.jpg "Western Blot: Nucleostemin Overexpression Lysate (Adult Normal) [NBL1-11183] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleostemin.")
![Western Blot: Nucleostemin Overexpression Lysate [NBP2-04539] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleostemin.](http://images.novusbio.com/fullsize/GNL3L-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04539-img0001.jpg "Western Blot: Nucleostemin Overexpression Lysate [NBP2-04539] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nucleostemin.")