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Human nSMase ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity HuSpecies Glossary
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.067 ng/mL

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Human nSMase ELISA Kit (Colorimetric) Summary

Description
Assay time: 3 hours
Standard Curve Range
0.156-10 ng/mL
Sensitivity
0.067 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12
Intra-Assay
%CV < 10
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SMPD2

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent A
  2. Assay Diluent B
  3. Detection Reagent A
  4. Detection Reagent B
  5. Instruction manual
  6. Plate sealer for 96 wells
  7. Pre-coated ready to use 96-well strip plate
  8. Standard Diluent
  9. Standard
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (30 x concentrate)

Alternate Names for Human nSMase ELISA Kit (Colorimetric)

  • EC 3.1.4.12
  • ISC1
  • lyso-PAF-PLC
  • Lyso-platelet-activating factor-phospholipase C
  • Neutral sphingomyelinase
  • NSMASE
  • N-SMase
  • NSMASE1
  • sphingomyelin phosphodiesterase 2
  • sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)

Background

Sphingomyelin is also present in all eukaryotic cell membranes, especially the plasma membrane, and is particularly concentrated in the nervous system because sphingomyelin is a major component of myelin, the fatty insulation wrapped around nerve cells by Schwann cells or oligodendrocytes. Multiple Sclerosis is a disease characterised by deterioration of the myelin sheath, leading to impairment of nervous conduction. nSMase (Neutral sphingomyelinase; Sphingomyelin phosphodiesterase 2) converts sphingomyelin to ceramide. Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain. Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SMPD2