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nSMase Antibody [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-97998AF350
Alexa Fluor 405
NBP2-97998AF405
Alexa Fluor 488
NBP2-97998AF488
Alexa Fluor 532
NBP2-97998AF532
Alexa Fluor 594
NBP2-97998AF594
Alexa Fluor 647
NBP2-97998AF647
Alexa Fluor 700
NBP2-97998AF700
Alexa Fluor 750
NBP2-97998AF750
Allophycocyanin
NBP2-97998APC
Biotin
NBP2-97998B
DyLight 350
NBP2-97998UV
DyLight 405
NBP2-97998V
DyLight 488
NBP2-97998G
DyLight 550
NBP2-97998R
DyLight 594
NBP2-97998DL594
DyLight 650
NBP2-97998C
DyLight 680
NBP2-97998FR
DyLight 755
NBP2-97998IR
FITC
NBP2-97998F
HRP
NBP2-97998H
Janelia Fluor 525
NBP2-97998JF525
Janelia Fluor 549
NBP2-97998JF549
Janelia Fluor 646
NBP2-97998JF646
Janelia Fluor 669
NBP2-97998JF669
PE
NBP2-97998PE
PerCP
NBP2-97998PCP
Unconjugated
NBP2-97998-100ul
mFluor Violet 450 SE
NBP2-97998MFV450
mFluor Violet 500 SE
NBP2-97998MFV500
mFluor Violet 610 SE
NBP2-97998MFV610
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nSMase Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Produced in rabbits immunized with E. coli-derived Human nSMase fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMPD2
Purity
Antigen and protein A Affinity-purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Antigen and protein A Affinity-purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for nSMase Antibody [CoraFluor™ 1]

  • EC 3.1.4.12
  • ISC1
  • lyso-PAF-PLC
  • Lyso-platelet-activating factor-phospholipase C
  • Neutral sphingomyelinase
  • NSMASE
  • N-SMase
  • NSMASE1
  • sphingomyelin phosphodiesterase 2
  • sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)

Background

Sphingomyelin is also present in all eukaryotic cell membranes, especially the plasma membrane, and is particularly concentrated in the nervous system because sphingomyelin is a major component of myelin, the fatty insulation wrapped around nerve cells by Schwann cells or oligodendrocytes. Multiple Sclerosis is a disease characterised by deterioration of the myelin sheath, leading to impairment of nervous conduction. nSMase (Neutral sphingomyelinase; Sphingomyelin phosphodiesterase 2) converts sphingomyelin to ceramide. Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain. Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB5018
Western Blot PLA2G1B Antibody (630823)
Species: Hu
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nSMase Antibody
[CoraFluor™ 1]
NBP2-97998CL1
nSMase Antibody [CoraFluor™ 1]
Species: Hu
Applications: IHC

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Bioinformatics

Gene Symbol SMPD2