| Reactivity | MuSpecies Glossary |
| Applications | Flow, CyTOF-ready |
| Clone | 605724 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2 Leu26-Val528 (predicted) Accession # O35516 |
| Specificity | Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100%
cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity
with rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinant
rat (rr) Notch2, or rrDLL1 is observed. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | NOTCH2 |
| Purity Statement | Protein A or G purified from hybridoma culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins. It is found on multiple cell types and exhibits multiple functions. On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation. Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470). Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor. There are three potential splice variants. One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713. Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.
![Notch-2 Antibody (605724) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Notch-2 Antibody (605724) [Unconjugated]")
| Publication using MAB5196 | Applications | Species |
|---|---|---|
| Meah, A;Vedarethinam, V;Bronstein, R;Gujarati, N;Jain, T;Mallipattu, SK;Li, Y;Wang, J; Single-Cell Spatial MIST for Versatile, Scalable Detection of Protein Markers Biosensors 2023-08-27 [PMID: 37754086] (IHC, Mouse) | IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
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