Notch-2 Antibodies
We offer Notch-2 Antibodies for use in common research applications: Blockade of Receptor-ligand Interaction, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Western Blot. Each Notch-2 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Notch-2 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Notch-2 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Notch-2 polyclonal antibodies and browse our Notch-2 monoclonal antibody catalog.
Alternate Names for Notch-2 Antibodies
anti-Notch-2 antibody, anti-NOTCH2 antibody, anti-AGS2 antibody, anti-hN2 antibody, anti-neurogenic locus notch homolog protein 2 antibody, anti-Notch (Drosophila) homolog 2 antibody, anti-notch 2Notch homolog 2 (Drosophila) antibody, anti-Notch homolog 2 antibody
14 Results for "notch-2" in Primary Antibodies
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP2-27347-img0002.jpg "Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP1-19125-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Notch2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP1-19125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody [NBP2-48913] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48913-img0004.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody [NBP2-48913] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP2-48913] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP2-48913-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B6-Chimeric-Immunofluorescence-NBP2-62559-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B6) - Chimeric [NBP2-62559] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B6. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B6 (NBP2-62559) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62559, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Notch-2-Antibody-B9-Chimeric-Immunofluorescence-NBP2-62560-img0001.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody (B9) - Chimeric [NBP2-62560] - Immunofluoresence staining of fixed HeLa cells with anti-Notch 2 antibody B9. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of B9 (NBP2-62560) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-62560, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")