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Notch-2 Antibody (411801) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
411801
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB3735-SP
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Notch-2 Antibody (411801) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Notch‑2
Asp2063-Pro2413
Accession # Q04721.3
Specificity
Detects human Notch-2 intracellular domain (ICD) in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) Notch-1 ICD, recombinant rat (rr) Notch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch‑4 ICD.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
NOTCH2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Notch-2 Antibody (411801) [Unconjugated]

  • AGS2
  • hN2
  • neurogenic locus notch homolog protein 2
  • Notch (Drosophila) homolog 2
  • notch 2Notch homolog 2 (Drosophila)
  • Notch homolog 2
  • Notch2
  • Notch-2

Background

Notch-2 is a 300 kDa member of the Notch family of transmembrane (TM) proteins.  It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180 kDa disulfide-linked extracellular domain (ECD) (likely aa 25-1538) and a 110 kDa membrane bound truncated segment (likely aa 1539-2471).  Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa 1697-2471), a potential nuclear transcription factor.  In the nucleus, NICD regulates CSL pathways.  When NICD is phosphorylated, it is deactivated.  Over aa 2063-2413, human Notch-2 is 94% and 89% aa identical to dog and mouse Notch-2, respectively.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NOTCH2
Uniprot