Reactivity | MuSpecies Glossary |
Applications | Flow |
Clone | 605724 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Alexa Fluor 700 |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Immunogen | Chinese hamster ovary cell line CHO-derived recombinant mouse Notch-2 Leu26-Val528 (predicted) Accession # O35516 |
Specificity | Detects mouse Notch-2 in direct ELISAs. In direct ELISAs, 50-100%
cross-reactivity with recombinant human (rh) Notch-2 and no cross-reactivity
with rhNotch-1, -2, -3, recombinant mouse (rm) Notch-1, rmNotch-3, recombinant
rat (rr) Notch2, or rrDLL1 is observed. |
Isotype | IgG1 |
Clonality | Monoclonal |
Host | Rat |
Gene | NOTCH2 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative | 0.09% Sodium Azide |
Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
Notch-2 (also Motch-2) is a 280-300 kDa member of the notch family of transmembrane (TM) proteins. It is found on multiple cell types and exhibits multiple functions. On CD8+ T cells, it augments cytotoxic activity; on pre-B cells, it drives B1 B cell development; on endothelial cells, it induces apoptosis; and on proerythroblasts, it promotes proliferation. Mature mouse Notch-2 is a 2445 amino acid (aa) type I TM glycoprotein. It undergoes Golgi processing to generate a 180-200 kDa extracellular domain (ECD) (aa 26-1606) possibly covalently-linked to a 100-110 kDa membrane bound C-terminal segment (aa 1607-2470). Upon binding to Delta1, Jagged1, or Jagged2, the 110 kDa segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) that serves as a potential nuclear transcription factor. There are three potential splice variants. One shows a 32 aa substitution for aa 293-2470, a second contains a 13 aa substitution for aa 1-1954, and a third possesses a five aa substitution for aa 224-227 coupled to an 11 aa substitution for aa 1704-1713. Over aa 26-528, mouse Notch-2 shares 96% and 94% aa identity with rat and human Notch-2, respectively.
Secondary Antibodies |
Isotype Controls |
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