Notch-1 Antibody - (Cleaved N terminal)

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.

Showing Publications 1 - 9 of 9.

Publications using NB300-251	Applications	Species
Han J, Hyun J, Park J et al. Aberrant role of pyruvate kinase M2 in the regulation of gamma-secretase and memory deficits in Alzheimer's disease Cell reports 2021-12-07 [PMID: 34879266]
Bhardwaj M, Sharma A, Sen S et al. Chlamydia and ocular adnexal lymphomas: An Indian experience Exp. Mol. Pathol. 2016-07-17 [PMID: 27435913] (WB, Human)	WB	Human
Lee HA, Park S, Kim Y. Effect of beta-carotene on cancer cell stemness and differentiation in SK-N-BE(2)C neuroblastoma cells. Oncol Rep 2013-10-01 [PMID: 23900747]
Park S, Kim J, Kim Y. Mulberry leaf extract inhibits cancer cell stemness in neuroblastoma Nutr Cancer 2012-08-01 [PMID: 22860924] (WB, Human)	WB	Human
Sciacca S, Pilato M, Mazzoccoli G et al. Anti-correlation between longevity gene SirT1 and Notch signaling in ascending aorta biopsies from patients with bicuspid aortic valve disease Heart Vessels 2012-02-28 [PMID: 22370592] (WB, Human)	WB	Human
Wang J, Zhang Y, Wei J et al. Effect of Notch activation on the regenerative response to acute renal failure. Am J Physiol Renal Physiol 2010-01-01 [PMID: 19828677]
Solomon A, Mian Y, Ortega-Cava C et al. Upregulation of the let-7 microRNA with precocious development in lin-12/Notch hypermorphic Caenorhabditis elegans mutants. Dev Biol 2008-04-01 [PMID: 18334253]
Stylianou S, Clarke RB, Brennan K et al. Aberrant activation of notch signaling in human breast cancer. Cancer Res 2006-02-01 [PMID: 16452208]
Milano, J et al. Modulation of Notch Processing by gamma-Secretase Inhibitors Causes Intestinal Goblet Cell Metaplasia Induction of Genes Known to Specify Gut Secretory Lineage Differentiation. Toxicological Sciences. [PMID: 15319485]

Images	Ratings	Applications	Species	Date	Details
Enlarge	1 reviewed by: Ram Kuwar	WB	Rat	10/23/2020	View Summary Application Western Blot Sample Tested Rat brain tissue Species Rat Lot 42173 Comments Comments ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Array NB300-251

• Notch-1 Antibodies
• Notch-1 ELISA Kits
• Notch-1 Lysate
• Notch-1 Proteins
• Notch-1 Proteins and Enzymes
• Notch-1 ELISA