![Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP2-19563-img0013.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human non-muscle Myosin IIA. The exact sequence is proprietary. |
| Predicted Species | Mouse (98%), Rat (97%), Chicken (96%), Canine (99%), Xenopus (92%), Bovine (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYH9 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 227 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-muscle Myosin IIA antibody detects Non-muscle Myosin IIA protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Non-muscle Myosin IIA stained by Non-muscle Myosin IIA antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19563-img0009.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-muscle Myosin IIA antibody detects Non-muscle Myosin IIA protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Non-muscle Myosin IIA stained by Non-muscle Myosin IIA antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP2-19563] - Human adenocarcinoma, using MYH9 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP2-19563-img0002.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP2-19563] - Human adenocarcinoma, using MYH9 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - A. 30 ug U87-MG whole cell lysate/extract. B. 30 ug SK-N-SH whole cell lysate/extract. C. 30 ug IMR32 whole cell lysate/extract. D. 30 ug SK-N-AS whole cell lysate/extract.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP2-19563-img0003.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - A. 30 ug U87-MG whole cell lysate/extract. B. 30 ug SK-N-SH whole cell lysate/extract. C. 30 ug IMR32 whole cell lysate/extract. D. 30 ug SK-N-AS whole cell lysate/extract.")
![Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - A. 30 ug NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP2-19563-img0005.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - A. 30 ug NT2D1 whole cell lysate/extract B. 30 ug PC-3 whole cell lysate/extract 5 % SDS-PAGE MYH9 antibody [N1], N-term dilution: 1:1000")
![Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Western-Blot-NBP2-19563-img0011.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP2-19563] - Imunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1], N-term. Western blot analysis was performed using MYH9 antibody [N1], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunoprecipitation-NBP2-19563-img0007.jpg "Immunoprecipitation: non-muscle Myosin IIA Antibody [NBP2-19563] - Imunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 ug of MYH9 antibody [N1], N-term. Western blot analysis was performed using MYH9 antibody [N1], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19563_rabbit-polyclonal-non-muscle-myosin-iia-antibody-1610202419405738.jpg "Western Blot: non-muscle Myosin IIA Antibody [NBP2-19563] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1], N-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")