![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Analysis in human endometrium and skeletal muscle tissues using NBP1-85244 antibody. Corresponding MYH9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0012.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Analysis in human endometrium and skeletal muscle tissues using NBP1-85244 antibody. Corresponding MYH9 RNA-seq data are presented for the same tissues.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: REQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDF |
| Predicted Species | Mouse (95%), Rat (94%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MYH9 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB reported in scientific literature (PMID: 24155382). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using NBP1-85244 | Applications | Species |
|---|---|---|
| Sun Y, Qi Y, Liu C et al. Nonmuscle Myosin Heavy Chain IIA Is a Critical Factor Contributing to the Efficiency of Early Infection of Severe Fever with Thrombocytopenia Syndrome Virus. J Virol 2014-01-01 [PMID: 24155382] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for non-muscle Myosin IIA Antibody (NBP1-85244)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MYH9 |
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-MYH9 antibody NBP1-85244 (A) shows similar protein distribution across tissues to independent antibody NBP2-62644 (B).](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0010.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-MYH9 antibody NBP1-85244 (A) shows similar protein distribution across tissues to independent antibody NBP2-62644 (B).")
![Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85244-img0009.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and actin filaments. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0017.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human skeletal muscle shows no positivity in myocytes as expected.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0013.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human endometerium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0014.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human endometerium shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle fibers and in endothelial cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0015.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle fibers and in endothelial cells.")
![Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining in human testis shows strong cytoplasmic and membranous positivity in Leydig cells.](http://images.novusbio.com/fullsize/non-muscle-Myosin-IIA-Antibody-Immunohistochemistry-Paraffin-NBP1-85244-img0016.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody [NBP1-85244] - Staining in human testis shows strong cytoplasmic and membranous positivity in Leydig cells.")
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![Western Blot Uromodulin Antibody [Unconjugated]](https://images.novusbio.com/images2/Uromodulin_AF5175_Western_Blot_5648.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")