![Western Blot: NOL12 Antibody [NBP2-19560] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NOL12-Antibody-Western-Blot-NBP2-19560-img0002.jpg "Western Blot: NOL12 Antibody [NBP2-19560] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NOL12. The exact sequence is proprietary. |
| Localization | Nucleus; nucleolus |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NOL12 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 25 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
![Intracellular Staining by Flow Cytometry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Flow_Cytometry_11328.jpg)
![Simple Western Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin1_AF1152_Simple_Western_16673.jpg)
![Immunohistochemistry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Immunohistochemistry_6684.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: NOL12 Antibody [NBP2-19560] - Analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19560-img0004.jpg "Immunocytochemistry/Immunofluorescence: NOL12 Antibody [NBP2-19560] - Analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. NOL12 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0007.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. NOL12 antibody dilution: 1:500.")
![Western Blot: NOL12 Antibody [NBP2-19560] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NOL12-Antibody-Western-Blot-NBP2-19560-img0001.jpg "Western Blot: NOL12 Antibody [NBP2-19560] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0005.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.")
![Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunohistochemistry-Paraffin-NBP2-19560-img0006.jpg "Immunohistochemistry-Paraffin: NOL12 Antibody [NBP2-19560] - NOL12 antibody detects NOL12 protein at nucleous on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NOL12 antibody dilution: 1:500.")
![Western Blot ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Western_Blot_5097.jpg)
![Immunohistochemistry ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Immunohistochemistry_20696.jpg)
![Knockout Validated ERK2 Antibody [Unconjugated]](https://images.novusbio.com/images2/ERK2_AF1230_Knockout_Validated_22864.jpg)
![Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Flow_Cytometry_17077.jpg)
![Immunocytochemistry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Immunocytochemistry__Immunofluorescence_17398.jpg)
![Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]](https://images.novusbio.com/images2/Integrin_beta_1_MAB17781_Flow_Cytometry_23861.jpg)
![Western Blot Galectin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin3_AF1197_Western_Blot_21471.jpg)
![Immunohistochemistry Galectin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-3_AF1197_Immunohistochemistry_7115.jpg)
![Simple Western Galectin-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin3_AF1197_Simple_Western_18971.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")