![Western Blot: NOL12 Antibody [NBP1-46174] - Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody NBP1-46174 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/NOL12-Antibody-Western-Blot-NBP1-46174-img0004.jpg "Western Blot: NOL12 Antibody [NBP1-46174] - Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody NBP1-46174 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen for this product maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NOL12 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NOL12 |
![Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (NBP1-46174) Detection: DAB](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0005.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (NBP1-46174) Detection: DAB")
![Western Blot: NOL12 Antibody [NBP1-46174] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody NBP1-46173, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/NOL12-Antibody-Western-Blot-NBP1-46174-img0001.jpg "Western Blot: NOL12 Antibody [NBP1-46174] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody NBP1-46173, which recognizes an upstream epitope.")
![Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0003.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .")
![Immunoprecipitation: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOL12 antibody A302-733A (lot A302-733A-2) used for IP at 3 ug per reaction. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody A302-732A. For blotting immunoprecipitated NOL12, A302-733A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/NOL12-Antibody-Immunoprecipitation-NBP1-46174-img0006.jpg "Immunoprecipitation: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NOL12 antibody A302-733A (lot A302-733A-2) used for IP at 3 ug per reaction. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody A302-732A. For blotting immunoprecipitated NOL12, A302-733A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")