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NMDA R, NR2A Subunit Antibody [Unconjugated]

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Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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NMDA R, NR2A Subunit Antibody [Unconjugated] Summary

Immunogen
Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor
Specificity
Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2A
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:1000 to 1:2000 dilution
  • Immunoprecipitation
  • Western Blot 1:1000 dilution
Publications
Read Publications using
PPS012 in the following applications:

Packaging, Storage & Formulations

Storage
After reconstitution the antibody should be aliquoted and stored at ≤ -20° C. This product is stable at ≤- 20° C for at least 1 year.
Buffer
Lyophilized from 5 mM ammonium bicarbonate
Preservative
No Preservative
Reconstitution Instructions
This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR2A Subunit Antibody [Unconjugated]

  • EPND
  • FESD
  • GluN1
  • GluN2A
  • GRIN2A
  • LKS
  • MRD8
  • NDHMSD
  • NDHMSR
  • NMDA R, NR2A Subunit
  • NMDA1
  • NMDAR1
  • NMDAR2A
  • NMD-R1
  • NR1
  • NR2A

Background

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMDA R, NR2A Subunit Antibody (PPS012)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
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Filter By Species
Human
(1)
Mouse
(1)
Rat
(1)
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Showing Publications 1 - 6 of 6.
Publications using PPS012 Applications Species
HF Jiao, XD Sun, R Bates, L Xiong, L Zhang, F Liu, L Li, HS Zhang, SQ Wang, MT Xiong, M Patel, AM Stranahan, WC Xiong, BM Li, L Mei Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus Proc. Natl. Acad. Sci. U.S.A, 2017-01-17;114(5):1177-1182. 2017-01-17 [PMID: 28096412] (Western Blot, Human) Western Blot Human
Xiang-Dong Sun Lrp4 in astrocytes modulates glutamatergic transmission Nat Neurosci, 2016-06-13;0(0):. 2016-06-13 [PMID: 27294513] (Western Blot, Mouse) Western Blot Mouse
C.M. Forrest, K. McNair, M. Pisar, O.S. Khalil, L.G. Darlington, T.W. Stone Altered hippocampal plasticity by prenatal kynurenine administration, kynurenine-3-monoxygenase (KMO) deletion or galantamine Neuroscience 2015-12-03 [PMID: 26365611]
Sofya Abazyan, Eun Ju Yang, Bagrat Abazyan, Meng Xia, Chunxia Yang, Camilo Rojas et al. Mutant disrupted-in-schizophrenia 1 in astrocytes: Focus on glutamate metabolism Journal of Neuroscience Research 2014-12-01 [PMID: 25131692]
C.M. Forrest, O.S. Khalil, M. Pisar, K. McNair, E. Kornisiuk, M. Snitcofsky et al. Changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway Neuroscience 2013-12-19 [PMID: 24076085]
Forrest , Caroline, Khalil , Omari S, Pisar , Mazura, Smith , Robert A, Darlington , Lynda Ga, Stone , Trevor W Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring. Mol Brain, 2012-06-09;5(0):22. 2012-06-09 [PMID: 22681877] (Western Blot, Rat) Western Blot Rat

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WB Video Protocol

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Bioinformatics

Gene Symbol GRIN2A