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NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free

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Western Blot: NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free [NBP2-79873] - Western Blot Analysis of PC-3 cell lysate using NM23-H2/NME2 Antibody (CPTC-NME2-2).
Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free [NBP2-79873] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with NM23-H2/NME2 Antibody (CPTC-NME2-2).
Protein Array: NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free [NBP2-79873] - Analysis of Protein Array containing more than 19,000 full-length human proteins using NM23-H2/NME2 Antibody (CPTC-NME2-2). Z- and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, MA
Clone
CPTC-NME2-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79738).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant full-length human NM23-H2/NME2 protein (Uniprot: P22392)
Localization
Cytoplasmic, Nuclear
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NME2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for NM23-H2/NME2 Antibody (CPTC-NME2-2) - Azide and BSA Free

  • EC 2.7.13.3
  • Histidine protein kinase NDKB
  • MGC2212
  • NDK B
  • NDKB
  • NDP Kinase B2
  • NDPKB
  • NDPK-B
  • NM23B
  • NM23-H2
  • NME2
  • non-metastatic cells 2, protein (NM23B) expressed in
  • nucleoside diphosphate kinase B
  • protein (NM23) expressed in

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NME2