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NM23-H1 Antibody

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Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody [NBP1-30884] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry: NM23-H1 Antibody [NBP1-30884] - NM23-H1 antibody [C1C3] detects NM23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ...read more
Genetic Strategies: Western Blot: NM23-H1 Antibody [NBP1-30884] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 ...read more

Product Details

Summary
Reactivity Hu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

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NM23-H1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NM23-H1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Rat (93%), Canine (93%), Primate (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NME1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (93%) and Chimpanzee (93%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for NM23-H1 Antibody

  • EC 2.7.4.6
  • GAAD
  • Granzyme A-activated DNase
  • Metastasis inhibition factor nm23
  • NB
  • NBS
  • NDK A
  • NDKA
  • NDP kinase A
  • NDPKA
  • NDPK-A
  • NM23A
  • NM23AWD
  • NM23H1
  • NM23-H1
  • NME1
  • non-metastatic cells 1, protein (NM23A) expressed in
  • nucleoside diphosphate kinase A
  • Tumor metastatic process-associated protein

Background

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NME1