NM23-H1 Antibody Summary
Immunogen |
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NM23-H1. The exact sequence is proprietary. |
Localization |
Cytoplasm, Nucleus |
Predicted Species |
Rat (93%), Canine (93%), Primate (93%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
NME1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:100-1:1000
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:100-1:1000
- Western Blot 1:500-1:3000
|
Theoretical MW |
17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (93%) and Chimpanzee (93%).
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol |
Preservative |
0.01% Thimerosal |
Purity |
Antigen Affinity-purified |
Alternate Names for NM23-H1 Antibody
Background
The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
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