NM23-H1 Antibodies
We offer NM23-H1 Antibodies for use in common research applications: Dot Blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each NM23-H1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NM23-H1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the NM23-H1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NM23-H1 polyclonal antibodies and browse our NM23-H1 monoclonal antibody catalog.
Alternate Names for NM23-H1 Antibodies
anti-NM23-H1 antibody, anti-NME1 antibody, anti-EC 2.7.4.6 antibody, anti-GAAD antibody, anti-Granzyme A-activated DNase antibody, anti-Metastasis inhibition factor nm23 antibody, anti-NB antibody, anti-NBS antibody, anti-NDK A antibody, anti-NDKA antibody, anti-NDP kinase A antibody, anti-NDPK-A antibody, anti-NDPKA antibody, anti-NM23AWD antibody, anti-non-metastatic cells 1, protein (NM23A) expressed in antibody, anti-nucleoside diphosphate kinase A antibody, anti-Tumor metastatic process-associated protein antibody
17 Results for "nm23-h1" in Primary Antibodies
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0011.jpg "Western Blot: NME1-NME2 Antibody [NBP1-80992] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: NME1-NME2 Antibody [NBP1-80992] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80992-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myoctes.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0014.jpg)
![Western Blot: NME1-NME2 Antibody [NBP1-80992] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Western-Blot-NBP1-80992-img0007.jpg)
![Immunohistochemistry-Paraffin: NME1-NME2 Antibody [NBP1-80992] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/NME1-NME2-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0009.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0012.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody [NBP1-80992] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunohistochemistry-Paraffin-NBP1-80992-img0013.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0014.jpg "Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody (M02), clone 1D7.Lane 1: NME1 transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunocytochemistry-Immunofluorescence-H00004830-M02-img0007.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunohistochemistry-Paraffin-H00004830-M02-img0009.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in HeLa.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0012.jpg)
![Western Blot: NM23-H1 Antibody (1D7) [H00004830-M02] - NME1 monoclonal antibody (M02), clone 1D7. Analysis of NME1 expression in different cell lines.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Western-Blot-H00004830-M02-img0013.jpg)
![Immunoprecipitation: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of NME1 transfected lysate using anti-NME1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NME1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-Immunoprecipitation-H00004830-M02-img0010.jpg)
![ELISA: NM23-H1 Antibody (1D7) [H00004830-M02] - Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-1D7-ELISA-H00004830-M02-img0011.jpg)
![Western Blot: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Western-Blot-NBP2-46186-img0011.jpg "Western Blot: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.")
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0010.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0001.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0002.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0003.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0004.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0005.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0006.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0007.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0008.jpg)
![Immunohistochemistry: NM23-H1 Antibody (4H2) [NBP2-46186] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4H2-Immunohistochemistry-NBP2-46186-img0009.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-278202415212429.jpg "Western Blot: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 ug/Lane) Lane 2: PC-3M cell lysate (20 ug/Lane) Lane 3: HeLa cell lysate (20 ug/Lane) Lane 4: HepG2 cell lysate (20 ug/Lane) Lane 5: A549 cell lysate (20 ug/Lane) Lane 4: NIH/3T3 cell lysate (20 ug/Lane) Lane 5: C6 cell lysate (20 ug/Lane) Lane 5: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/19 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32656) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32656) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-298202414333679.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-32656) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202410484818.jpg)
![Flow Cytometry: NM23-H1 Antibody (12A1-R) [NBP3-32656] - Flow cytometric analysis of MCF7 cells labeling NM23-H1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32656, 1μg/mL) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32656_mouse-nm23-h1-mab-12a1-r-29202416372150.jpg)
![Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Western-Blot-NBP1-30884-img0006.jpg "Western Blot: NM23-H1 Antibody [NBP1-30884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody (NBP1-30884). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP1-30884] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30884-img0002.jpg)
![Immunohistochemistry: NM23-H1 Antibody [NBP1-30884] - NM23-H1 antibody [C1C3] detects NM23-H1 protein at cytosol on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. NM23-H1 antibody [C1C3] (NBP1-30884) dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-30884_rabbit-polyclonal-nm23-h1-antibody-910202420115083.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP1-30884] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30884_rabbit-polyclonal-nm23-h1-antibody-1610202419414579.jpg)
![Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: HeLa cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: A431 cell lysate (20 ug/Lane) Lane 9: HepG2 cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: C6 cell lysate (20 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Mouse liver tissue lysate (40 ug/Lane) Lane 14: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-28820241423318.jpg "Western Blot: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Raji cell lysate (20 ug/Lane) Lane 5: HeLa cell lysate (20 ug/Lane) Lane 6: K-562 cell lysate (20 ug/Lane) Lane 7: A549 cell lysate (20 ug/Lane) Lane 8: A431 cell lysate (20 ug/Lane) Lane 9: HepG2 cell lysate (20 ug/Lane) Lane 10: NIH/3T3 cell lysate (20 ug/Lane) Lane 11: C6 cell lysate (20 ug/Lane) Lane 12: Mouse kidney tissue lysate (40 ug/Lane) Lane 13: Mouse liver tissue lysate (40 ug/Lane) Lane 14: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33132) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33132) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-29820241492692.jpg)
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C2-R) [NBP3-33132] - Immunocytochemistry analysis of HeLa cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33132) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33132_mouse-nm23-h1-mab-13c2-r-2920241192324.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-Western-Blot-NBP2-19549-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NM23-H1 Antibody [NBP2-19549] - Wild-type (WT) and nm23-H1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with nm23-H1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody [NBP2-19549] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19549-img0003.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0001.jpg)
![Western Blot: NM23-H1 Antibody [NBP2-19549] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/nm23-H1-Antibody-Western-Blot-NBP2-19549-img0002.jpg)
![Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-Western-Blot-NBP2-13662-img0004.jpg "Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)")
![Immunohistochemistry-Paraffin: NM23-H2/NME2 Antibody [NBP2-13662] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal and non-germinal center cells.](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-Immunohistochemistry-Paraffin-NBP2-13662-img0005.jpg)
![Western Blot: NM23-H2/NME2 Antibody [NBP2-13662] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/NM23-H2-NME2-Antibody-Western-Blot-NBP2-13662-img0003.jpg)
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0004.jpg "Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLane 5 : MCF7 cell lysateLane 6 : PC3 cell lysate")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (5F4) [NBP2-59498] - Analysis of NME1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of NME1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NME1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Immunocytochemistry-Immunofluorescence-NBP2-59498-img0002.jpg)
![Western Blot: NM23-H1 Antibody (5F4) [NBP2-59498] - Lane 1: NME1, Lane 2: NME2 Lane 3: NME3 Lane 4: NME4](http://images.novusbio.com/fullsize/NM23-H1-Antibody-5F4-Western-Blot-NBP2-59498-img0001.jpg)
![Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-288202414191531.jpg "Western Blot: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Western blot analysis of NM23-H1 on different lysates with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: HepG2 cell lysate Lane 6: K-562 cell lysate Lane 7: A549 cell lysate Lane 8: Raji cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 17 kDa Observed band size: 17/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33131) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: NM23-H1 Antibody (13C1-R) [NBP3-33131] - Immunocytochemistry analysis of MCF7 cells labeling NM23-H1 with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-NM23-H1 antibody (NBP3-33131) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33131_mouse-nm23-h1-mab-13c1-r-2920241192318.jpg)