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NM23-H1 Antibody (OTI4H2) - Azide and BSA Free

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Western Blot: NM23-H1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-73003] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.
Western Blot: NM23-H1 Antibody (OTI4H2) - Azide and BSA Free [NBP2-73003] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NM23-H1.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73003AF350
Alexa Fluor 405
NBP2-73003AF405
Alexa Fluor 488
NBP2-73003AF488
Alexa Fluor 532
NBP2-73003AF532
Alexa Fluor 594
NBP2-73003AF594
Alexa Fluor 647
NBP2-73003AF647
Alexa Fluor 700
NBP2-73003AF700
Alexa Fluor 750
NBP2-73003AF750
Allophycocyanin
NBP2-73003APC
Biotin
NBP2-73003B
CoraFluor 1
NBP2-73003CL1
DyLight 350
NBP2-73003UV
DyLight 405
NBP2-73003V
DyLight 488
NBP2-73003G
DyLight 550
NBP2-73003R
DyLight 594
NBP2-73003DL594
DyLight 650
NBP2-73003C
DyLight 680
NBP2-73003FR
DyLight 755
NBP2-73003IR
FITC
NBP2-73003F
HRP
NBP2-73003H
Janelia Fluor 525
NBP2-73003JF525
Janelia Fluor 549
NBP2-73003JF549
Janelia Fluor 646
NBP2-73003JF646
Janelia Fluor 669
NBP2-73003JF669
PE
NBP2-73003PE
PerCP
NBP2-73003PCP
Unconjugated
NBP2-46186
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46186
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NM23-H1 Antibody (OTI4H2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human NME1 (NP_937818) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
NME1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for NM23-H1 Antibody (OTI4H2) - Azide and BSA Free

  • EC 2.7.4.6
  • GAAD
  • Granzyme A-activated DNase
  • Metastasis inhibition factor nm23
  • NB
  • NBS
  • NDK A
  • NDKA
  • NDP kinase A
  • NDPKA
  • NDPK-A
  • NM23A
  • NM23AWD
  • NM23H1
  • NM23-H1
  • NME1
  • non-metastatic cells 1, protein (NM23A) expressed in
  • nucleoside diphosphate kinase A
  • Tumor metastatic process-associated protein

Background

The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Knockout Validated Nbs1 Antibody
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Bioinformatics

Gene Symbol NME1