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NIR2 Antibody

Images

 
Western Blot: NIR2 Antibody [NBP2-34132] - TEST
Immunocytochemistry/ Immunofluorescence: NIR2 Antibody [NBP2-34132] - Staining of human cell line A549 shows positivity in cytoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NIR2 Antibody [NBP2-34132] - Staining of human Pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.
Western Blot: NIR2 Antibody [NBP2-34132] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Western Blot: NIR2 Antibody [NBP2-34132] - Analysis in human cell line HDLM-2.
Immunohistochemistry-Paraffin: NIR2 Antibody [NBP2-34132] - Staining of human fallopian tube shows cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: NIR2 Antibody [NBP2-34132] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: NIR2 Antibody [NBP2-34132] - Staining of human Fallopian tube shows strong cytoplasmic positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: NIR2 Antibody [NBP2-34132] - Staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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NIR2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: PKEMTKWNSNDFIDAFASPVEAEGTPEPGAEAAKGIEDGAQAPRDSEGLDGAGELGAEACAVHALFLILHSGNILDSGP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PITPNM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1:100 - 1:250
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NIR2 Protein (NBP2-34132PEP)
Publications
Read Publication using NBP2-34132.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NIR2 Antibody

  • DRES9NIR-2
  • Drosophila retinal degeneration B homolog
  • FLJ44997
  • membrane-associated phosphatidylinositol transfer protein 1
  • NIR2PITPNM
  • phosphatidylinositol transfer protein, membrane-associated 1Pyk2 N-terminal domain-interacting receptor 2
  • PITPnm 1
  • Rd9
  • RDGB
  • RDGB1
  • RDGBA
  • RDGBA1
  • retinal degeneration B alpha 1

Background

NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NIR2 Antibody (NBP2-34132)(1)

Reviews for NIR2 Antibody (NBP2-34132) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NIR2 Antibody (NBP2-34132) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional NIR2 Products

Array NBP2-34132

Research Areas for NIR2 Antibody (NBP2-34132)

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Blogs on NIR2

There are no specific blogs for NIR2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

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Contact Information

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Bioinformatics

Gene Symbol PITPNM1
Uniprot