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Nicastrin Antibody (716918)

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Nicastrin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Nicastrin Monoclonal Antibody (Catalog # MAB5378) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF
Clone
716918
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Nicastrin Antibody (716918) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Nicastrin
Asn34-Glu669
Accession # Q92542
Specificity
Detects human Nicastrin in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCSTN
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB5378 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nicastrin Antibody (716918)

  • anterior pharynx-defective 2
  • APH2
  • ATAG1874
  • KIAA0253APH2
  • NCSTN
  • Nicastrin

Background

Nicastrin (NCT) is a 150‑160 kDa member of the Nicastrin family of proteins. It is a component of the aspartyl protease gamma -secretase complex and serves to stabilize and direct gamma -secretase components to proper positions in the plasma membrane. The gamma -secretase complex mediates the cleavage of intramembrane proteins such as Notch-1 and APP. Mature human Nicastrin is a 676 amino acid type I transmembrane glycoprotein. It contains a 636 aa extracellular domain (aa 34‑669) that shows a 58 aa sequence (aa 312‑369) which interacts with gamma -secretase substrates. There are multiple splice variants of NCT. One shows a deletion of aa 195‑322 and 394‑709, a second shows a 29 aa substitution for the C-terminal 604 aa and a third shows a deletion of aa 200‑709 accompanied by an insertion of 33 aa after Leu30. Over aa 34‑669, human NCT shares 90% aa identity with mouse NCT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nicastrin Antibody (MAB5378) (0)

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Review for Nicastrin Antibody (MAB5378) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB5378:
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ICC
(1)
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  5
reviewed by:
Verified Customer
ICC Human 03/22/2022
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Summary

ApplicationImmunocytochemistry
Sample TestedHepG2 human hepatocellular carcinoma cell line
SpeciesHuman
Lot716918

Comments

CommentsIncubated at 10 ug/mL for 3 hours at room temperature

FAQs for Nicastrin Antibody (MAB5378) (0)

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Verified Customer
03/22/2022
Application: ICC
Species: Human

Bioinformatics

Gene Symbol NCSTN
Uniprot