![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826] - Staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NCSTN monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-004-Immunocytochemistry-Immunofluorescence-NBP2-89826-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826] - Staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human NCSTN monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF |
| Clone | 004 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | This antibody was obtained from a rabbit immunized with purified, recombinant Human Nicastrin (Accession#: NP_056146.1; Met1-Glu669). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | NCSTN |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Nicastrin Antibody (NBP2-89826)Find related products by research area.
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| Gene Symbol | NCSTN |
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