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NFkB2/NFkB p100 Antibody (291319) [Unconjugated]

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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). For additional reference, lysates of Raji human Burkitt's lymphoma cell line were included. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for NF kappa B2 were detected at approximately 52 kDa and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human CD40 Ligand/TNFSF5 aa 108-261 (Catalog # 6245-CL) for 4 hours. Gels were loaded with ...read more
Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. NF kappa ...read more
NF kappa B2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human NF kappa B2 Monoclonal Antibody (Catalog # MAB28881) at 3 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clone
291319
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB28881-SP
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NFkB2/NFkB p100 Antibody (291319) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human NF kappa B2
Met1-Asn447
Accession # Q00653
Specificity
Detects human NFkB2 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with endogenous mouse NFkB2 in NIH 3T3 cells is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFKB2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB28881 in the following applications:
Publications
Read Publication using MAB28881.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFkB2/NFkB p100 Antibody (291319) [Unconjugated]

  • CVID10;H2TF1;LYT10;LYT-10;NF-kB2;Nuclear factor NF-kappa-B p100 subunit;p100;p49/p100;p52
  • LYT10
  • NFkB2
  • NF-kB2

Background

Nuclear Factor kappa B2 (NF kappa B2 or NF kappa B p52) is a member of the NF kappa B/Rel family of transcription factors. NF kappa B2 dimerizes with other members of the NF kappa B/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NFkB2/NFkB p100 Antibody (MAB28881)(1)


Review for NFkB2/NFkB p100 Antibody (MAB28881) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using MAB28881:
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Verified Customer
 WB 12/31/2023
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Summary

ApplicationWestern Blot
LotYSY0322041

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Western Blot NFkB2/NFkB p100 MAB28881
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 Verified Customer
12/31/2023
Application: WB
Species:
read this review

Bioinformatics

Gene Symbol NFKB2
Uniprot