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NFkB p105/p50 Transcription Factor Assay Kit (Colorimetric)

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NFkB p105/p50 Assay Kit (Colorimetric) [KA1339] - Sensitivity of the NF-?B assay using purified human recombinant p50.

Product Details

Summary
Applications Func
Suitable Sample Type
Nuclear extracts

Order Details

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NFkB p105/p50 Transcription Factor Assay Kit (Colorimetric) Summary

Description
NF-kB (human p50) Transcription Factor Assay Kit is a non-radioactive method for detecting specific transcription factor DNA binding activity.
Assay Type
Colorimetric
Sample Volume
10 uL/well. All samples should be assayed in duplicate at minimum.
Kit Type
Transcription Factor Assay Kit (Colorimetric)
Gene
NFKB1

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 96-Well Cover Sheet
  2. Polysorbate 20
  3. Transcription Factor Antibody Binding Buffer (10X)
  4. Transcription Factor Binding Assay Buffer (4X)
  5. Transcription Factor Developing Solution
  6. Transcription Factor Goat Anti-Rabbit HRP Conjugate
  7. Transcription Factor NF-?B (p50) Primary Antibody
  8. Transcription Factor NF-kB (human p50) Positive Control 1
  9. Transcription Factor NF-kB 96-Well Strip Plate
  10. Transcription Factor NF-kB Competitor dsDNA
  11. Transcription Factor Reagent A
  12. Transcription Factor Stop Solution
  13. Wash Buffer Concentrate (400X)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NFkB p105/p50 Transcription Factor Assay Kit (Colorimetric)

  • DKFZp686C01211
  • DNA binding factor KBF1
  • DNA-binding factor KBF1
  • EBP-1
  • KBF1
  • NF-kappaB
  • NF-kappa-B
  • NF-kappabeta
  • NFKB-p105
  • NFKB-p50
  • nuclear factor kappa-B DNA binding subunit
  • nuclear factor NF-kappa-B p105 subunit
  • nuclear factor NF-kappa-B p50 subunit
  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
  • p105
  • p50

Background

NF-kB (human p50) Transcription Factor Assay Kit is a non-radioactive method for detecting specific transcription factor DNA binding activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Blogs on NFkB p105/p50.

Nuclear Factor kappa B Signaling in the Immune System
Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed...  Read full blog post.

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Bioinformatics

Gene Symbol NFKB1