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NFATC2/NFAT1 Antibody

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Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NFATC2/NFAT1 Antibody [NBP1-51970] - NBP1-51970 (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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NFATC2/NFAT1 Antibody Summary

Immunogen
Peptide with sequence C-QQFRTGLSSPDARYQ corresponding to internal region according to NP_775114.1, NP_001129493.1.
Epitope
C-QQFRTGLSSPDARYQ
Specificity
This antibody is expected to recognize all three reported isoforms (NP_036472.2; NP_775114.1; NP_001129493.1).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NFATC2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human MOLT4 lysates (calculated MW of 100 kDa band according to NP_775114.1). IHC-P: Human spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes.
Publications
Read Publication using NBP1-51970.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NFATC2/NFAT1 Antibody

  • NFAT pre-existing subunit
  • NFAT transcription complex, preexisting component
  • NFAT1
  • NFAT1nuclear factor of activated T-cells, cytoplasmic 2
  • NFATC2
  • NF-ATc2
  • NFATP
  • NF-ATp
  • NFATPnuclear factor of activated T-cells, preexisting component
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • preexisting nuclear factor of activated T-cells 2
  • T cell transcription factor NFAT1
  • T-cell transcription factor NFAT1

Background

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NFATC2/NFAT1
Antibody
NBP1-51970
NFATC2/NFAT1 Antibody
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, ELISA, IHC

Publications for NFATC2/NFAT1 Antibody (NBP1-51970)(1)


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Bioinformatics

Gene Symbol NFATC2
Entrez
Uniprot