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NFATC2/NFAT1 Antibody (25A10.D6.D2) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, GS, ICC/IF, IHC, IP, ChIP
Clone
25A10.D6.D2
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB300-504AF350
Alexa Fluor 405
NB300-504AF405
Alexa Fluor 488
NB300-504AF488
Alexa Fluor 532
NB300-504AF532
Alexa Fluor 594
NB300-504AF594
Alexa Fluor 647
NB300-504AF647
Alexa Fluor 700
NB300-504AF700
Alexa Fluor 750
NB300-504AF750
Biotin
NB300-504B
DyLight 350
NB300-504UV
DyLight 405
NB300-504V
DyLight 488
NB300-504G
DyLight 550
NB300-504R
DyLight 594
NB300-504DL594
DyLight 650
NB300-504C
DyLight 680
NB300-504FR
DyLight 755
NB300-504IR
FITC
NB300-504F
HRP
NB300-504H
Janelia Fluor 525
NB300-504JF525
Janelia Fluor 549
NB300-504JF549
Janelia Fluor 646
NB300-504JF646
Janelia Fluor 669
NB300-504JF669
Unconjugated
NB300-504
mFluor Violet 450 SE
NB300-504MFV450
mFluor Violet 500 SE
NB300-504MFV500
mFluor Violet 610 SE
NB300-504MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB300-504
BSA Free
NB300-504-0.025mg
BSA Free
NB300-504
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NFATC2/NFAT1 Antibody (25A10.D6.D2) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms.
Specificity
Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NFATC2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Gel Super Shift Assays
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NFATC2/NFAT1 Antibody (25A10.D6.D2) [CoraFluor™ 1]

  • NFAT pre-existing subunit
  • NFAT transcription complex, preexisting component
  • NFAT1
  • NFAT1nuclear factor of activated T-cells, cytoplasmic 2
  • NFATC2
  • NF-ATc2
  • NFATP
  • NF-ATp
  • NFATPnuclear factor of activated T-cells, preexisting component
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • preexisting nuclear factor of activated T-cells 2
  • T cell transcription factor NFAT1
  • T-cell transcription factor NFAT1

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1, NFAT2, NFAT3, and NFAT4. These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NFATC2/NFAT1
Antibody (25A10.D6.D2 ...
NB300-504CL1
NFATC2/NFAT1 Antibody (25A10.D6.D2) [CoraFluor™ 1]
Species: Hu, Mu, Rt
Applications: WB, ELISA, GS, ICC/IF, IHC, IP, ChIP

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Bioinformatics

Gene Symbol NFATC2