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NF-H Antibody (RT97) - Azide and BSA Free

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Western Blot: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Western Blot Analysis of human brain tissue lysate using NF-H Antibody (RT97).
Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded Rat Cerebellum stained with NF-H Antibody (RT97).
Immunohistochemistry-Paraffin: NF-H Antibody (RT97) - Azide and BSA Free [NBP2-33062] - Formalin-fixed, paraffin-embedded human Cerebellum stained with NF-H Antibody (RT97).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChSpecies Glossary
Applications WB, Flow, IHC
Clone
RT97
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

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NF-H Antibody (RT97) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29435).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Triton-X 100 insoluble protein fraction of rat brain (Uniprot: P12036)
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NEFH
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-33062 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for NF-H Antibody (RT97) - Azide and BSA Free

  • 200 kDa neurofilament protein
  • KIAA0845
  • NEFH
  • Neurofilament Heavy (200kDa)
  • neurofilament heavy polypeptide
  • Neurofilament triplet H protein
  • neurofilament, heavy polypeptide 200kDa
  • neurofilament, heavy polypeptide
  • NF200
  • NFH
  • NF-H

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-33062
Species: Hu, Mu, Rt, Po, Ch
Applications: WB, Flow, IHC

Publications for NF-H Antibody (NBP2-33062)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Product General Protocols

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Secondary Antibodies

 

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Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

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Bioinformatics

Gene Symbol NEFH