![Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Western-Blot-NB300-136-img0002.jpg "Western Blot: NF-H Antibody (NAP4) [NB300-136] - Analysis of tissue lysates using mouse mAb to NF-H, NB300-136, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord, [7] cow spinal cord. Strong band at about 200-220 kDa corresponds to the major phosphorylated from of the NF-H subunit. A minor band at about 160 kDa is the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with NB300-136 antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, EqSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | NAP4 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Native NF-H purified from bovine spinal cord |
| Localization | Cytoplasmic |
| Marker | Neuronal Marker |
| Specificity | Specifically recognizes the phosphorylated variant of NF-H subunit (~200-220 kDa), showing some weaker reactivity with phosphorylated forms of NF-M. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NEFH |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This 200-220 kDa Neurofilament Heavy antibody can be used for ELISA, Immunohistochemistry on paraffin-embedded sections and Western blot. |
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| Theoretical MW | 200-220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% PBS, 50% glycerol |
| Preservative | 0.035% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Affinity purified |
| Publication using NB300-136 | Applications | Species |
|---|---|---|
| Jasmin, J-F, Malhotra, S, Singh Dhallu, M, Mercier, I, Rosenbaum, D M, Lisanti, M P. Caveolin-1 Deficiency Increases Cerebral Ischemic Injury, . 100, 721-729. 2007-01-01 [PMID: 17293479] (IHC-P, Rat) | IHC-P | Rat |
Secondary Antibodies |
Isotype Controls |
Research Areas for NF-H Antibody (NB300-136)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
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![Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.](http://images.novusbio.com/fullsize/NF-H-Antibody-NAP4-Immunohistochemistry-NB300-136-img0001.jpg "Immunohistochemistry: NF-H Antibody (NAP4) [NB300-136] - Analysis of human cerebellar cortex section stained with mouse mAb to pNF-H, NB300-136, in brown. Paraffin-embedded, formalin-fixed tissue sections were stained with this antibody using the avidin biotin conjugate method. The sections was counterstained with Hematoxylin in blue. NB300-136 stains prominent basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image, the molecular layer at the top.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")