![Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:1000.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Western-Blot-NBP3-12972-img0001.jpg "Western Blot: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:1000.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Nesfatin-1/Nucleobindin-2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | NUCB2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Nesfatin-1/Nucleobindin-2 Antibody (NBP3-12972)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | NUCB2 |
![Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nesfatin-1/Nucleobindin-2 protein stained by Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-12972-img0003.jpg "Immunocytochemistry/Immunofluorescence: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nesfatin-1/Nucleobindin-2 protein stained by Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Immunohistochemistry-Paraffin-NBP3-12972-img0002.jpg "Immunohistochemistry-Paraffin: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Nesfatin-1/Nucleobindin-2 antibody detects Nesfatin-1/Nucleobindin-2 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunoprecipitation: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Immunoprecipitation of Nesfatin-1/Nucleobindin-2 protein from 293T whole cell extracts using 5 ug of Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972). Western blot analysis was performed using Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Nesfatin-1-Nucleobindin-2-Antibody-Immunoprecipitation-NBP3-12972-img0004.jpg "Immunoprecipitation: Nesfatin-1/Nucleobindin-2 Antibody [NBP3-12972] - Immunoprecipitation of Nesfatin-1/Nucleobindin-2 protein from 293T whole cell extracts using 5 ug of Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972). Western blot analysis was performed using Nesfatin-1/Nucleobindin-2 antibody (NBP3-12972) diluted at 1:500. EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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